oppA [2017-8-11 16:0:3]

oligopeptide ABC transporter (binding protein)

Locus: BSU11430

Isoelectric point: 5.72

Molecular weight: 61.32 kDa

Protein length: 545 aa

Gene length: 1635 bp

Function: initiation of sporulation, competence development

Product: oligopeptide ABC transporter (binding protein)

Essential: no

Synonyms: spo0KA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of peptides
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of peptides
• Information processing → Genetics → Genetic competence
• Information processing → Regulation of gene expression → phosphorelay
• Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
• Lifestyles → Sporulation → phosphorelay
• Groups of genes → Secreted proteins
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue
• Groups of genes → Phosphoproteins → Phosphorylation on a Tyr residue

Gene

Coordinates: 1,219,849 → 1,221,486

The protein

Catalyzed reaction/ biological activity

• required for the uptake of quorum sensing peptides such as PhrH PubMed

Protein family

• bacterial solute-binding protein 5 family (according to Swiss-Prot)

Paralogous protein(s)

• DppE

Modification

• phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 PubMed

Structure

• 1OLA (from Salmonella enterica, 36% identity) PubMed

Localization

• attached to the cell membrane (via OppB-OppC) PubMed
• at the outer side of the cell: extracellular (signal peptide) PubMed, lipid modification as retention signal PubMed

Expression and Regulation

Operons

• Genes: oppA-oppB-oppC-oppD-oppF
  Description: PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon
  • CodY: repression, no derepression occurs, however, in a CodY mutant, due to increased repression by ScoC PubMed, in CodY regulon
  • ScoC: repression, PubMed, in ScoC regulon

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Additional information

  • A ncRNA is predicted between 'trpS' and 'oppA' PubMed

Biological materials

Mutant

• BP67 (spc) available in Fabian Commichau's lab
• 1S118 ( oppA::spec), PubMed, available at BGSC
• GP2097 (D(oppA-oppB-oppC-oppD-oppF)::aphA3), available in Jörg Stülke's lab
• BKE11430 (oppA::erm, available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed

References

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